ABSTRACT
Objective To analyze the influencing factors of extra uterine growth retardation (EUGR) in premature very low birth weight infants.Methods A total of 61 EUGR premature very low birth weight infants survived in intensive care unit of our hospital were enrolled into EUGR group,and at the same time another 50 non-EUGR premature very low birth weight infants were selected into non-EUGR group.The clinical related medical records in the two groups were compared and analyzed,and the influencing factors of EUGR was analyzed by Logistic regression analysis.Results The birth gestational age of EUGR group was (31.29±1.56)weeks,which was significantly lower than (33.98±1.35)weeks of the non-EUGR group (t=9.60,P<0.05).The birth weight of the EUGR group was (1 206.76±212.14)g,which was significantly lower than (1 341.55±103.26)g of the non-EUGR group (t=4.11,P<0.05).The hospital stay,return to birth weight time,start enteral nutrition time and total parenteral nutrition time in the EUGR group were (27.77±5.00)d,(15.36±5.91)d,(3.36±1.91)d,(16.93±4.02)d respectively,which were significantly longer than those in the non-EUGR group[22.69±3.97)d,(10.61±4.57)d,(2.61±1.37)d,(9.43±3.11)d](t=3.53,4.65,2.33,10.80,all P<0.05).The incidence rates of complications such as respiratory system,digestive system and metabolic disorder in the EUGR group were 26.23%,19.67% and 67.21%,respectively,which were significantly higher than those of the non-EUGR group(8.00%,6.00% and 40.00%)(x2=6.18,4.39,8.22,all P<0.05).Logistic regression analysis showed that low birth gestational age and birth weight,long hospital stay,later return to birth weight and total parenteral nutrition time,intrauterine growth retardation,and respiratory system,digestive system and metabolic disorder were independent risk factors for the occurrence of EUGR(all P<0.05).Conclusion The influencing factors of EUGR in very low birth weight infants were mainly low gestational age and birth weight,long hospital stay,later return to birth weight and total parenteral nutrition time,intrauterine growth retardation,and respiratory system,digestive system and metabolic disorder.Therefore,in order to prevent EUGR,active symptomatic measures should be given in clinic.
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Objective To establish a pretreatment mthod of the tissue sample of rat and a determination method of the concentration of isorhynchophylline in tissue of rats by RP-HPLC. Method The tissue sample were treated by adding methanol, and the supernatant was dried by nitrogen gas, the residue was redissolved in methanol, then detected by RP-HPLC directly. Waters? RP18 (4.6 mm?250 mm, 5 ?m) and pre-column Wondasil C18 (4.0 mm?10 mm, 5 ?m) were used as the stationary phase. Methanol-water (70∶30) added 0.03% triethylamine was taken as mobile phase with flow rate of 0.8 mL/min. The wavelength of detection was 254 nm. Results The standard curve of Isorhynchophylline concentraton in rat tissues:Y=48.558X+0.207 6 (n=6), r=0.999 6, it appears a good linear relationship within the range of 0.16～16.0 ?g/mL. The average recovery of was 99.66%, RSD was 1.56%. Conclusion This mehod is sensitive, rapid, simple, specific and reproducible.
ABSTRACT
【Objective】 To identify the main factor or factors w hich contribute to recurrent early spontaneous abortion for clinical diagnosis a nd treatment. 【Methods】 69 cases received the screening examination including inherent, endocrine, anatomical, infective, immunological and contamination hist ory poison metabolic factors, then analyses all factors. 【Results】 Analysing the single factor effecting th e recurrent early spontaneous abortion in the above 7 factors, we found the immun ological factor was 86.2%. Furthermore the immunological factor had significant difference in the various aged groups. 【Conclusion】 Immunological factor is a main factor in recurrent early spontaneous abortion. The immunological factor b ecomes significantly important as the times of spontaneous abortion increasing.